Journal: Vaccine
Article Title: Retinoic acid-adjuvanted vaccine induces antigen-specific secretory IgA in the gut of newborn piglets.
doi: 10.1016/j.vaccine.2024.126672
Figure Lengend Snippet: Fig. 5. Retinoic acid did not induce significantly higher cell mediated IFN- γ or IL17 responses. Cell mediated IFN-γ (A) and IL17 (B) levels (geometric mean with SD) in supernatants of CTH522 re-stimulated PBMCs measured by ELISA. SEB stimulated PBMCs are included as positive control (C, D). Back ground cytokine production from non-stimulated samples was subtracted from the antigen re-stimulated samples in (A, B) and values ≤0 were set to 1. There was no statistical significance compared to the o/w-CTH522 group as deter mined by one-way ANOVA followed by Dunnett’s multiple comparisons test (GraphPad Prism version 10.1.2). All values were log-transformed prior to statistical analysis.
Article Snippet: For IL-17 MaxiSorp plates were coated with rabbit anti-pig IL-17A (KP0498S-100, Kingfisher Biotech, 2.5 mg/mL) and developed with biotinylated rabbit anti-pig IL17A antibody (KPB0499S-050, Kingfisher Biotech, 100 ng/mL) and HRP-conjugated streptavidin 1:10000.
Techniques: Enzyme-linked Immunosorbent Assay, Positive Control, Transformation Assay